2/19/2023 0 Comments Chip seq from flashfrozen cells![]() Super-long-read DNA sequencing: For this protocol we shear the DNA to fragments of about 50 kb lengths using the MegaRuptor then ligate-on the sequencing adapters.Yields per PromethION flowcell can range from 20 Gb up to 130 Gb. Long-read DNA sequencing: of sheared DNA fragments from 5 kb to 20 kb.We offer different types of PromethION sequencing: We have achieved the best run metrics mentioned below with DNA isolated from human/mammalian cell cultures and mammalian blood samples. Average yields will be inversely correlated to the library molecule lengths (insert sizes). The average sequence data yields can vary widely and will mostly depend on the sample properties (please see below). We now offer HMW-DNA isolation as a service (currently for animal samples) since high quality single-molecule sequencing data depend on highest quality DNA samples. Depending on the samples and the library types, the PromethION can generate up to 100 Gb of sequence data per flow cell, and can run up to 24 flow cells simultaneously. To our knowledge we are the first academic core lab to offer such services. ![]() We offer Nanopore sequencing on the highest throughput Nanopore sequencer, the PromethION. To learn more about the technology, please see the HOW-IT-WORKS page and video below. The Nanopore sequencing data greatly enable de novo genome assemblies and structural genomic variant and transcriptome studies. For high molecular weight DNA (HMW-DNA) samples, read lengths of several hundred kb can be reached with ultra-long-read protocols. Nanopore sequencing with Oxford Nanopore Technologies (ONT) systems enables high-throughput long-read sequencing of both DNA and RNA samples.
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